Validation of Candidate Genes
Leaf amino acid profile changes in response to N among B73 x IHP1, B73 x ILP1, and B73 x Mo17 hybrids. The transport and storage amino acid asparagine (Asn) increases relative to metabolically active glutamine (Gln) in response to N and the IHP genotype.
The metabolic pahtway for asparagine accumulation.
B73 and IHP1 plants were grown in hydroponics under either Ndeficient (1 mM NO3 -) or Nsufficient (14 mM NO3 -) conditions. Leaves were sampled from these plants during both the day and night. RNA was isolated and the relative expression of genes encoding an asparagine synthetase (AS2) or asparaginase isoform was assayed by qRT-PCR.
Quantitative Real-Time PCR
analysis of NitroGenes:
AS2 RNA expression
increases in response to N
and is also elevated in IHP1
relative to B73, particularly
during the day. Conversely,
asparaginase RNA
expression is repressed by
N, and is greatly reduced in
IHP1 compared to B73.
